Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: duodenum epithelium was single-cell digestedand FACS sorted for positive cells and similar number of negative cells. RNA was extracted using microplus RNeasy kit (Qiagen) and quality checked on a picochip bioanalyzer (RIN >7) Library was constructed with 2ng of total RNA using the Ovation RNAseq system v2 kit and the Ovation Rapid DR multiplex 1-96 kit (Nugen)