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SRX4124266: GSM3154441: duodenum_NeuroD1_negative_6361; Mus musculus; RNA-Seq
2 ILLUMINA (Illumina HiSeq 4000) runs: 6.1M spots, 305.3M bases, 104.5Mb downloads

Submitted by: NCBI (GEO)
Study: Mouse duodenal enteroendocrine transcriptome
PRJNA473085 • SRP148960 • All experiments • All runs
show Abstracthide Abstract
Using genetically modifed mice to express fluorescent reporter in proglucagon expressing cells (GluVenus) or in all enteroendocrine cells (NeuroD1), we purifed positive and negative cells by FACS from duodenal epithelial cells and identified transcripts enriched in positive populations Overall design: Comparative transcriptomics between positive and negative cells in two different mice strains, with three replicates (2 for the GluVenus negative population)
Sample: duodenum_NeuroD1_negative_6361
SAMN09259628 • SRS3338727 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: duodenum epithelium was single-cell digestedand FACS sorted for positive cells and similar number of negative cells. RNA was extracted using microplus RNeasy kit (Qiagen) and quality checked on a picochip bioanalyzer (RIN >7) Library was constructed with 2ng of total RNA using the Ovation RNAseq system v2 kit and the Ovation Rapid DR multiplex 1-96 kit (Nugen)
Experiment attributes:
GEO Accession: GSM3154441
Links:
Runs: 2 runs, 6.1M spots, 305.3M bases, 104.5Mb
Run# of Spots# of BasesSizePublished
SRR72174162,881,830144.1M48.7Mb2019-01-01
SRR72174173,224,521161.2M55.8Mb2019-01-01

ID:
5609827

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